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KMID : 0896220070270010013
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Journal of Daegu Health College
2007 Volume.27 No. 1 p.13 ~ p.0
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Comparison between microwave fixation and chemical fixation for preparing biological tissue
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Ahn Seung-Ju
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Abstract
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Heat treatment causes partial denaturation of protein, which is one of basic principles of histological fixation. Application of conventional heating method like boiling is limited to small specimens due to its poor heat conduction in biological materials. Microwave heating method could be a good alternative to the boiling method. Microwave energy can be used for biological sample fixation, and is known to be as effective as or better than chemical fixation. In this study, the optimum condition for the fixation of rat kidney with microwave was obtained at 62±2℃(in 10 ㎖ N.B.F, 13 sec irradiation with a domestic microwave). The microwaved specimens were embedded in paraffin and sectioned. The quality of the section was improved in histochemical reaction when compared with the specimens fixed with other methods. The total duration engaged in the preparation with microwavefixed specimen was 16 hours, which is shorter than that of traditional methods.
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